Gene description for Hspa4
Gene name	heat shock protein family A (Hsp70) member 4
Gene symbol	Hspa4
Other names/aliases	Hsp110 Hsp70 irp94
Species	Rattus norvegicus
Experiment description of studies that identified Hspa4 in Pancreatic cancer cells
1 Experiment ID 93 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting Mass spectrometry [MALDI TOF] PubMed ID 20124479    Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zör M. Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS-Tspan8 Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting EV-TRACK EV100025: EV-METRIC:44%, 44%
2 Experiment ID 92 Identified molecule protein Extracellular vesicle type Exosomes MISEV EVs Identification method Western blotting PubMed ID 20124479    Organism Rattus norvegicus Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zör M. Journal name CR Publication year 2010 Sample Pancreatic cancer cells Sample name AS Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.14-1.17 g/mL Molecules identified in the study Protein mRNA Methods used in the study RT-PCR Western blotting RatRef-12 Microarray FACS Mass spectrometry [MALDI TOF] EV-TRACK EV100025: EV-METRIC:44%, 44%